Hepatities C Virus (HCV) is a significant health problem worldwide due to the lack of effective vaccines. HCV plasmid DNA (pDNA) vaccine represents a promising means to induce a Th1-biased cell-mediated response which tends to be associated with HCV clearance. However, the immune responses induced by naked pDNA vaccine in large animals as well as in humans are usually too weak to show sufficient protection against new infections. Therefore, it is interesting to look for new ways to deliver HCV pDNA vaccine. In this research, carbon nanotube (CNT) is used as a carrier to deliver the pDNA vaccine of HCV to induce high immune responses, because CNT has some excellent properties such as high strength and good biocompatibility. One of the key approaches to make this idea work is to treat CNT so that it can bind with HCV pDNA with good stability. An approach called 1, 3-dipolar cycloaddition of azomethine ylides was modified. We analyzed the complex of f-CNTs combined with pDNA vaccines expressing HCV E2 protein by using Enzyme-linked immunospot (ELISPOT) or Enzyme-linked immunosorbent assay (ELISA) assay in vitro. The result showed that the CNT approach can induce stronger protective immune responses than the needle delivery of naked pDNA vaccine. We have also found an optimal way to treat CNT in light of the highest immune response in the same testing environment. The success of this research will warrant testing HCV vaccine in large animal models and human clinical trials.
Skip Nav Destination
Article navigation
Design Of Medical Devices Conference Abstracts
A Novel Approach to Drug Delivery for Hepatities C Virus (HCV) for High Immune Responses
W. T. Chen,
W. T. Chen
University of Saskatchewan
, Saskatoon, Canada
Search for other works by this author on:
C. Zhang
C. Zhang
University of Saskatchewan
, Saskatoon, Canada
Search for other works by this author on:
W. T. Chen
University of Saskatchewan
, Saskatoon, Canada
C. Zhang
University of Saskatchewan
, Saskatoon, CanadaJ. Med. Devices. Jun 2008, 2(2): 027538 (1 pages)
Published Online: June 25, 2008
Article history
Published:
June 25, 2008
Citation
Chen, W. T., and Zhang, C. (June 25, 2008). "A Novel Approach to Drug Delivery for Hepatities C Virus (HCV) for High Immune Responses." ASME. J. Med. Devices. June 2008; 2(2): 027538. https://doi.org/10.1115/1.2932336
Download citation file:
561
Views
Get Email Alerts
Cited By
Related Articles
Structural Changes in Confined Lysozyme
J Biomech Eng (July,2009)
Peptide- and Aptamer-Functionalized Nanovectors for Targeted Delivery of Therapeutics
J Biomech Eng (July,2009)
Differential Translocation of Nuclear Factor-KappaB in a Cardiac Muscle Cell Line Under Gravitational Changes
J Biomech Eng (June,2009)
Nanofiber Covered Stent (NCS) for Vascular Diseases
J. Med. Devices (June,2008)
Related Chapters
Conclusions
Chitosan and Its Derivatives as Promising Drug Delivery Carriers
Advantages of Chitosan as Drug Delivery Systems
Chitosan and Its Derivatives as Promising Drug Delivery Carriers
Experimental Studies
Nanoparticles and Brain Tumor Treatment