..., with only E f significantly reduced. Refrigerating growth plate explants for less than 48 h maintains their maximal stress, equilibrium stress, matrix modulus, and permeability. However, cold storage at 4 °C for more than 48 h and freezing storage at −20 °C significantly alter the biomechanical response...

...A. Changoor; L. Fereydoonzad; A. Yaroshinsky; M. D. Buschmann 28 01 2011 18 02 2011 biological tissues biomechanics cellular biophysics electromechanical effects freezing refrigeration The percentage reduction in Ef due to 12 days of refrigeration at 4 ° C...

... procedures often necessitate a storage protocol, which may adversely affect the functional properties of cartilage. The effects of storage at either 4 ° C for periods of 6 days and 12 days, or during a single freeze-thaw cycle at − 20 ° C were examined in young bovine cartilage. Non-destructive...

...-thaw ECM microstructure is determined by the deformation of ETs during cryopreservation, freezing-induced deformation of ETs was measured with a newly developed quantum dot (QD)-mediated cell image deformetry system using dermal equivalents as a model tissue. The dermal equivalents were constructed...

... tissue is complicated by the extreme compliance of the tissue. The objectives of this study were to determine the effect of flash freezing on both specimen preparation and mechanical response and to quantify the effect of small deviations in specimen geometry on mechanical behavior. Specimens of porcine...

...Raghav Goel; Kyle Anderson; Joel Slaton; Franz Schmidlin; Greg Vercellotti; John Belcher; John C. Bischof Molecular adjuvants can be used to enhance the natural destructive mechanisms of freezing within tissue. This review discusses their use in the growing field of combinatorial or adjuvant...

... pump out cytotoxic drugs from the intracellular space. In this study, we tested a hypothesis that freezing and thawing (F/T) may enhance intracellular drug delivery to MDR cancer cells via F/T-induced denaturation of MDR-associated proteins and/or membrane permeabilization. After a human MDR cancer...

...Stéphanie Passot; Ioan Cristian Tréléa; Michèle Marin; Miquel Galan; G. John Morris; Fernanda Fonseca The freezing step influences lyophilization efficiency and protein stability. The main objective of this work was to investigate the impact on the primary drying stage of an ultrasound controlled...

...Ioan Cristian Trelea; Stéphanie Passot; Michèle Marin; Fernanda Fonseca Lyophilizing frozen pellets, and especially spray freeze-drying, have been receiving growing interest. To design efficient and safe freeze-drying cycles, local temperature and moisture content in the product bed have...

... is thus successfully predicted. The present approach suggests a highly efficient theoretical tool for probing into the freeze injury mechanisms of biological material due to ice formation during cryosurgery or cryopreservation process when external electric field was involved. 17 10 2008 25 04...

...Bumsoo Han; Jeffrey D. Miller; Jun K. Jung Freezing of biological tissue is emerging in various biomedical applications. The success of these applications requires precise control of the tissue functionality, which is closely associated with the microstructure of the extracellular matrix (ECM...

... to subzero temperatures at 5 ° C ∕ min . As the tissues were being frozen, a shape-independent differential scanning calorimeter technique was used to measure water loss from the tissues and, consequently, the best fit membrane permeability parameters ( L p g and E L p ) of ovarian tissues during freezing...

...M. Takahashi; S. Nomura; M. Jindai; S. Shibata; X. Zhu; Y. Watanabe; K. Kawachi; N. Okabe To minimize surgical stresses including blood loss and operation time to the patients during hepatic resection, we studied the feasibility of a combination of a partial liver freezing technique and shape...

...Daigo Tanaka; Kenji Shimada; Yoed Rabin Background : Cryosurgery is the destruction of undesired tissues by freezing, as in prostate cryosurgery, for example. Minimally invasive cryosurgery is currently performed by means of an array of cryoprobes, each in the shape of a long hypodermic needle...

... 07 October 2004 08 03 2005 biothermics cellular biophysics freezing mass transfer optical microscopes biological techniques The first practical light microscopes were invented and applied for laboratory studies of micro-organisms in the 1660’s by van Leeuwenhoek and Hooke...

...Loay Massalha; Avraham Shitzer The effects of a thermally-significant blood vessel, simulated by an embedded acrylic tube, 4.8 mm outer diameter, on the freezing field caused by a surface cryoprobe were studied experimentally in a tissue phantom. The flat, 15 mm diameter, circular cryoprobe...

... that greatly simplifies the prediction of the optimal rate of freezing of biological systems. Plot of the predicted optimal cooling rate ( B opt , ° C ∕ min ) as a function of water volume/surface area ( SA ∕ WV , μ m − 1 ) at different E L p values...

...Jiayao Zhang; George A. Sandison; Jayathi Y. Murthy; Lisa X. Xu A comprehensive computational framework to simulate heat transfer during the freezing process in prostate cancer cryosurgery is presented. Tissues are treated as nonideal materials wherein phase transition occurs over a temperature...

...Bumsoo Han; John C. Bischof Understanding the phase change behavior of biomaterials during freezing/thawing including their thermal properties at low temperatures is essential to design and improve cryobiology applications such as cryopreservation and cryosurgery. However, knowledge of phase change...

...Hiroshi Takamatsu, Associate Professor,; Norihiko Kumagae, Former Graduate Student, Recent studies show that during slow freezing of biological cells, the cells may be also injured by not only chemical damage but also mechanical damage induced by ice crystal compression. A new experimental...