Interstitium contains a matrix of fibrous molecules that creates considerable resistance to water and solutes in series with the microvessel wall. On the basis of our preliminary studies (Adamson et al., 1994, Microcirculation 1(4), pp. 251–265; Fu et al., 1995 Am. J. Physiol. 269(38), pp. H2124–H2140), by using laser-scanning confocal microscopy and a theoretical model for interstitial transport, we determined both microvessel solute permeability and solute tissue diffusion coefficient of -lactalbumin (Stokes radius ) from the rate of tissue solute accumulation and the radial concentration gradient around individually perfused microvessel in frog mesentery. is . for -lactalbumin is . This value of is comparable to that for small solute sodium fluorescein (Stokes radius ), while is only 3.4% of . Our results suggest that frog mesenteric tissue is much less selective to solutes than the microvessel wall.
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April 2005
Article
Determination of Microvessel Permeability and Tissue Diffusion Coefficient of Solutes by Laser Scanning Confocal Microscopy
Bingmei M. Fu,
Bingmei M. Fu
Department of Mechanical Engineering, Cancer Institute,
e-mail: fu@ccny.cuny.edu
University of Nevada
, Las Vegas, 4505 Maryland Parkway, Box 454027, Las Vegas, NV 89154
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Roger H. Adamson,
Roger H. Adamson
Department of Human Physiology, School of Medicine,
University of California at Davis
, Davis, CA 95616
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Fitz-Roy E. Curry
Fitz-Roy E. Curry
Department of Human Physiology, School of Medicine,
University of California at Davis
, Davis, CA 95616
Search for other works by this author on:
Bingmei M. Fu
Department of Mechanical Engineering, Cancer Institute,
University of Nevada
, Las Vegas, 4505 Maryland Parkway, Box 454027, Las Vegas, NV 89154e-mail: fu@ccny.cuny.edu
Roger H. Adamson
Department of Human Physiology, School of Medicine,
University of California at Davis
, Davis, CA 95616
Fitz-Roy E. Curry
Department of Human Physiology, School of Medicine,
University of California at Davis
, Davis, CA 95616J Biomech Eng. Apr 2005, 127(2): 270-278 (9 pages)
Published Online: September 18, 2004
Article history
Received:
May 22, 2003
Revised:
September 18, 2004
Citation
Fu, B. M., Adamson, R. H., and Curry, F. E. (September 18, 2004). "Determination of Microvessel Permeability and Tissue Diffusion Coefficient of Solutes by Laser Scanning Confocal Microscopy." ASME. J Biomech Eng. April 2005; 127(2): 270–278. https://doi.org/10.1115/1.1865186
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