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research-article

Saponins of Dioscorea Nipponicae inhibits IL-17A-induced changes in biomechanical behaviors of in vitro cultured human airway smooth muscle cells

[+] Author and Article Information
Yue Wang

Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, China; School of Pharmaceutical Engineering and Life Science&School of Nursing, Changzhou University, Changzhou, Jiangsu 213164, China
wangyuebj2003@cczu.edu.cn

Yifan Zhang

Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, China; School of Pharmaceutical Engineering and Life Science&School of Nursing, Changzhou University, Changzhou, Jiangsu 213164, China
biomech@yeah.net

Ming Zhang

Department of Thoracic and Cardiovascular Surgery, Changzhou No.2 People's Hospital, Affiliated to Nanjing Medical University, Changzhou, Jiangsu 213003, China
2867921952@qq.com

Jingjing Li

Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, China
jingjingli@cczu.edu.cn

Yan Pan

Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, China
py@cczu.edu.cn

Lei Liu

Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, China
bioll@cczu.edu.cn

Linhong Deng

Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, China; School of Pharmaceutical Engineering and Life Science&School of Nursing, Changzhou University, Changzhou, Jiangsu 213164, China
dlh@cczu.edu.cn

1Corresponding author.

ASME doi:10.1115/1.4042317 History: Received September 01, 2018; Revised December 03, 2018

Abstract

Airway hyperresponsiveness (AHR) is a hallmark of bronchial asthma, which is attributable to enhanced contraction of asthmatic airway smooth muscle (ASM). Although ß2 adrenergic receptor agonists are commonly used to relax ASM for treating AHR, there are side effects such as desensitization of long-term use. Therefore, it is desirable to develop alternative ASM relaxant, preferably based on natural products. One potential candidate is the inexpensive and widely available natural herb Saponins of Dioscorea Nipponicae (SDN) which has recently been reported to suppress the level of inflammatory factor IL-17A in ovalbumin-induced mice thereby alleviating the inflammation symptoms of asthma. Here we evaluated the biomechanical effect of SDN on IL-17A-mediated changes of cultured human airway smooth muscle cells (HASMCs) in vitro. The stiffness and traction force of the cells were measured by optical magnetic twisting cytometry (OMTC), Fourier transform traction force microscopy (FTTM), respectively, the cell proliferation was evaluated using MTT colorimetry, the cell migration was measured by cell scratch test, and the changes of cell cytoskeleton were assessed by laser confocal microscopy (LCM). We found that the stiffness and traction force of HASMCs were enhanced along with the increases of IL-17A concentration and exposure time, and SDN treatment dose-dependently reduced these IL-17A-induced changes in cell mechanical properties. Furthermore, SDN alleviated IL-17A-mediated effects on HASMCs proliferation, migration and cytoskeleton remodeling. These results demonstrate that SDN could potentially be a novel drug candidate as bronchodilator for treating asthma-associated AHR.

Copyright (c) 2018 by ASME
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