0
research-article

Bridging organ and cellular-level behavior in ex-vivo experimental platforms using populations of models of cardiac electrophysiology

[+] Author and Article Information
Carlos A. Ledezma

Department of Mechanical Engineering, University of College London, London, United Kingdom
c.ledezma@ucl.ac.uk

Benjamin Kappler

LifeTec Group, Eindhoven, Netherlands
b.kappler@lifetecgroup.com

Veronique Meijborg

Department of Medical Biology, Amsterdam UMC, Amsterdam, Netherlands
v.m.meijborg@amc.uva.nl

Bas Boukens

Department of Medical Biology, Amsterdam UMC, Amsterdam, Netherlands
b.j.boukens@amc.uva.nl

Marco Stijnen

LifeTec Group, Eindhoven, Netherlands
m.stijnen@lifetecgroup.com

PJ Tan

Department of Mechanical Engineering, University College London, London, United Kingdom
pj.tan@ucl.ac.uk

Vanessa Diaz-Zuccarini

Department of Mechanical Engineering, University College London, London, United Kingdom
v.diaz@ucl.ac.uk

1Corresponding author.

ASME doi:10.1115/1.4040589 History: Received May 16, 2018; Revised June 11, 2018

Abstract

The inability to discern between pathology and physiological variability is a key issue in cardiac electrophysiology since this prevents the use of minimally invasive acquisitions to predict early pathological behavior. The goal of this work is to demonstrate how experimentally-calibrated populations of models may be employed to inform which cellular-level pathologies are responsible for abnormalities observed in organ-level acquisitions whilst accounting for inter-subject variability; this will be done through an exemplary computational and experimental approach. Unipolar epicardial electrograms were acquired during an ex-vivo porcine heart experiment. A population of the ten Tusscher 2006 model was calibrated to activation-recovery intervals, measured from the electrograms, at three representative times. The distributions of the parameters from the resulting calibrated populations were compared to reveal statistically significant pathological variations. Activation-recovery interval reduction was observed in the experiments and the comparison of the calibrated populations of models suggested a reduced L-type calcium conductance and a high extra-cellular potassium concentration as the most probable causes for the abnormal electrograms. This behavior was consistent with a reduction in the cardiac output and was confirmed by other experimental measurements. A proof of concept method to infer cellular pathologies by means of organ-level acquisitions is presented, allowing for an earlier detection of pathology than would be possible with current methods. This novel method uses mathematical models as a tool for formulating hypotheses regarding the cellular causes of observed organ-level behaviors, whilst accounting for physiological variability has been unexplored.

Copyright (c) 2018 by ASME
Your Session has timed out. Please sign back in to continue.

References

Figures

Tables

Errata

Discussions

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In